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It is to be noted that 2.5 g/l of freeze-dried coffee is equal to one heaped teaspoonful, a quantity typically used in making a cup of coffee. Samples of the cold coffee mixtures were presented in 1.5 cm deep tissue culture bottles, which have a 5 cm2 clear face, illuminated by Artificial Daylight Fluorescent Lamps (D65) at 900 lux.

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